Fig. 5: Mouse models different aspects of early cell types. | Nature Neuroscience

Fig. 5: Mouse models different aspects of early cell types.

From: Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia

Fig. 5

a, Comparison of mouse forebrain clusters to primary progenitor cell types. Heatmap showing the comparison of mouse forebrain clusters from 16,053 cells to the primary progenitor populations identified in this dataset. Correlations are performed using Pearson correlations between cluster marker sets and identify that progenitor clusters 4 and 7 do not have a counterpart in mouse data. b, C1orf61 is widely expressed in early human but not early mouse progenitors. Fluorescent in situ hybridization of human samples at CS16 shows broad expression of C1orf61 (cyan) in progenitor cells labeled by SOX2 (red). Parallel in situ staining in TS16 mouse shows no C1orf61 expression. Scale bars, 50 μM. c, ID4 is widely expressed in early human but not early mouse progenitors. Immunostaining of human samples at CS16 shows broad expression of ID4 (cyan) in progenitor cells labeled by SOX2 (red). A parallel staining in TS16 mouse shows no ID4 expression. Scale bars, 50 μM. d, Violin plots of ID4 RNA expression across several CSs (human) and embryonic days (mouse). ID4 RNA expression in the human persists onward from CS13. However, ID4 RNA expression in the mouse peaks at E11.5 (TS20) and dissipates. For all panels, each sample was immunostained once.

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