Extended Data Fig. 4: Normal radial positioning and morphology of layer III neurons in M1 in PAE mice at P30.

a, The percentage of reporter⁻ (control and PAE) and reporter⁺ (PAE) neurons among electroporated neurons in equally divided bins 1 to 10 spanning the upper to lower part of the entire thickness of the cerebral cortex. No significant differences are found between the distribution patterns in each group; P < 0.2 by one-tailed Kolmogorov-Smirnov test (n=3 per group). Graph shows mean ± SEM. b, Representative tracing of the neuronal morphology in each group. (c-e) There are no significant morphological differences in reporter⁺ cortical neurons in PAE mice compared with reporter⁻ neurons in both control and PAE mice; Number of dendrites/neuron c, F(2,122) = 1.46, P = 0.24 (total), F(2,122) = 1.47, P = 0.23 (apical), F(2,122) = 0.84, P = 0.43 (basal); number of branches/neuron [reporter⁻ (control, PAE): n = 40 cells each, reporter⁺ (PAE): n = 45 cells] d, F(2,122) = 0.17, P = 0.84 (apical primary), F(2,122) = 0.31, P = 0.73 (apical secondary), F(2,122) = 0.86, P = 0.43 (apical tertiary), F(2,122) = 0.21, P = 0.81 (basal primary), F(2,122) = 2.19, P = 0.12 (basal secondary), F(2,122) = 1.13, P = 0.33 (basal tertiary); length of dendrites/neuron [reporter⁻ (control, PAE): n = 40 cells each, reporter⁺ (PAE): n = 45 cells] e, F(2,22) = 1.02, P = 0.38 (apical primary), F(2,22) = 0.26, P = 0.77 (apical secondary), F(2,22) = 0.20, P = 0.820 (apical tertiary), F(2,22) = 0.01, P = 0.99 (basal primary), F(2,22) = 0.79, P = 0.47 (basal secondary), F(2,22) = 0.29, P = 0.08 (basal tertiary), all by one-way ANOVA [reporter⁻ (control): n = 9 cells, reporter⁻ (PAE): n = 8 cells, reporter⁺ (PAE): n = 8 cells]. In box plots (c-e), the line within the box indicates the median, and the upper and lower edges of the box represent the 25th and 75th percentiles, respectively. The upper and lower whisker boundaries indicate the 10th and 90th percentiles, respectively, and dots indicate outliers.