Extended Data Fig. 7: Tamapin binding colocalizes with Kcnn2 protein.

Biotinylated Tamapin (or vehicle-only control) was injected i.p. to control and PAE mice at P30. The cortex was fixed 30 minutes later for staining of biotin (green) and Kcnn2 (red). (a) Labeling for biotin (arrowhead) in layer III in M1 shows the co-localization of Tamapin and Kcnn2 protein. (b) Many Kcnn2⁺ cells in layer III in M1 are co-labeled for biotin in biotinylated Tamapin-injected PAE mice; ***P < 0.0001 by two-tailed Mann-Whitney U test (n = 10 per group). In the box plot, the line within the box indicates the median, and the upper and lower edges of the box represent the 25th and 75th percentiles, respectively. The upper and lower whisker boundaries indicate the 10th and 90th percentiles, respectively, and dots indicate outliers.