Extended Data Fig. 8: Minimal binding of Tamapin to Kcnn1⁺ or Kcnn3⁺ cells.

a, Biotinylated Tamapin was injected i.p. to PAE mice at P30. The cortex was fixed 30 minutes later for staining of biotin (green) and Kcnn1, 2, or 3 (red) in the hilus, in which Kcnn1, 2, and 3 have distinct expression patterns. Insets show higher magnification views of the areas outlined by broken lines. b, The percentage of biotin-labeled cells among the cells that express Kcnn1, 2, or 3 in the hilus, showing specific binding of biotinylated Tamapin to Kcnn2⁺ cells. F(2,21) = 148.21, P < 0.0001 by one-way ANOVA, **P < 0.01 by Tukey test (n = 8 brains per group). In the box plot, the line within the box indicates the median, and the upper and lower edges of the box represent the 25th and 75th percentiles, respectively. The upper and lower whisker boundaries indicate the 10th and 90th percentiles, respectively, and dots indicate outliers.