Extended Data Fig. 3: Cre/Dre-dependent tdTomato transgenic lines used for projection analysis of POMCLepr+ and POMCGlp1r+ neurons in 2D sections.

a, Illustration of experimental mice and schematic diagram showing Dre- and Cre-dependent targeted expression of tdTomato in either POMCLepr+ or POMCGlp1r+ neurons. Excision of loxP and rox-flanked stop cassettes through recombination of both Cre and Dre drivers leads to tdTomato expression in the targeted subpopulation. b, Representative microscopic images of immunofluorescent staining against tdTomato and POMC in 12-week-old POMCDre LeprCre ROSA26rSrlSltdTomato and POMCDre Glp1rCre ROSA26rSrlSltdTomato in the anterior bed nucleus of the stria terminalis (BNST), the periaqueductal gray (PAG), the dorsomedial hypothalamic area (DMH) and the paraventricular nucleus of the hypothalamus (PVH). Scale bar represents 150 μm. 3 V = third ventricle, ac = anterior commissure, AQ = cerebral aqueduct. c-d, Quantification of POMC (c) and tdTomato fiber density (d) assessed as raw integrated density of immunofluorescent staining depicted in (b). c, n = 5 mice and a minimum of 3, 3, 2, 1section(s) per mouse were analyzed for the BNST, PVH, DMH and PAG. d, n = 5 mice and a minimum of 3,3,2,1 sections per mouse were analyzed for the BNST, PVH, DMH and PAG. In d and e, data are represented as mean ± SEM. *p ≤ 0.05. Further statistical details are given in Source data extended Fig. 2