Extended Data Fig. 9: Validation of the Dre-Cre dependent ROSA26lSlrSrEGFPL10a transgenic line. | Nature Neuroscience

Extended Data Fig. 9: Validation of the Dre-Cre dependent ROSA26lSlrSrEGFPL10a transgenic line.

From: Functionally distinct POMC-expressing neuron subpopulations in hypothalamus revealed by intersectional targeting

Extended Data Fig. 9

a-b, Southern blots of ROSA26lSlrSrEGFPL10a mouse line with the ROSA26 (a) and Neo probe (b). The asterisk indicates the clone chosen for blastocyst injection. c, Representative microscopic images of immunofluorescent staining against POMC and EGFP in the ARC of all resulting genotypes at 12 weeks of age. Scale bar represents 50 μm. 3 V = third ventricle. d, Principal component analysis of all RNA input and IP samples. e-g, GO term representations of the differentially enriched genes between the POMCLepr+ and POMCGlp1r+ clusters mapping the percentage of regulated genes to the total GO term gene count against significant gene count and adjusted p-value per term. h, UMAP plot of 773 POMC neurons identified in normal chow diet fed mice. Cells that are closer in position in the plot have a similar genetic profile. In (e-g) p-values were calculated using the DESeq2 1.26.0 pipeline with adjustments for multiple comparisons.

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