Fig. 6: Differential expression of endogenous mRNAs for identified candidates in POMCLepr+ and POMCGlp1r+ neurons. | Nature Neuroscience

Fig. 6: Differential expression of endogenous mRNAs for identified candidates in POMCLepr+ and POMCGlp1r+ neurons.

From: Functionally distinct POMC-expressing neuron subpopulations in hypothalamus revealed by intersectional targeting

Fig. 6

a, Representative microscopic images of RNA ISH against Pomc, Glp1r and Lepr together with differentially expressed neuropeptidergic signaling candidate RNAs identified in the ribotag experiments, Cartpt, Npy1r, Oprm1 and Nmur2, in C57BL/6N mice at 12 weeks of age. First image shows ISH in the ARC with nuclear counterstain (blue, DAPI). Magnifications of the boxes (right) are shown with the indicated stainings. Pomc-positive neurons are outlined in white. Yellow and cyan arrows indicate Lepr-positive or Glp1r-positive POMC neurons, respectively. Scale bars represent 100 μm in the merged image and 25 μm in the magnifications. b, Violin plots showing the quantified intensity of Pomc mRNA across the rostrocaudal axis of the ARC in POMCLepr+ or POMCGlp1r+ neurons as assessed from RNA ISH (Fig. 2a). Data are from n = 4 mice, with a minimum of four sections analyzed per animal. PomcRostral, Lepr+: Q1: 7.83, Q2: 31.12, Q3: 71.31; Glp1r+: Q1: 3.92, Q2: 14.86, Q3: 37.05, unpaired Mann–Whitney U-test, Lepr+ versus Glp1r+, U = 21,051, PuT < 0.0001; PomcMid, Lepr+: Q1: 3.02, Q2: 15.71, Q3: 49.22; Glp1r+, Q1: 3.22, Q2: 13.43, Q3: 34.06, unpaired Mann–Whitney U-test, Lepr+ versus Glp1r+, U = 53,910, PuT = 0.0796; PomcCaudal, Lepr+: Q1: 9.46, Q2: 28.3, Q3: 103.5; Glp1r+ Q1: 8.03, Q2: 17.81, Q3: 91.96, unpaired Mann–Whitney U-test, Lepr+ versus Glp1r+, U = 3,467, PuT = 0.06349. a.u., arbitrary units. c, Violin plots showing quantified expression of the RNA-seq candidates measured as integrated density in POMCLepr+ or POMCGlp1r+ neurons as assessed from RNA ISH (a). Cartpt: Lepr+, Q1: 2,977, Q2: 6,729, Q3: 13,244, Glp1r+, Q1: 2,405, Q2: 4,741, Q3: 9,198, Lepr+ versus Glp1r+, UMW = 9,252, PuT = 0.0182; Npy1r: Lepr+, Q1: 1,609, Q2: 2,470, Q3: 3,887; Glp1r+, Q1: 1,154, Q2: 1,802, Q3: 3,069, Lepr+ versus Glp1r+, U = 22,352, PuT < 0.0001; Oprm1: Lepr+, Q1: 631.6, Q2: 800.8, Q3: 1,046; Glp1r+, Q1: 729.2, Q2: 1,002, Q3: 1,360, Lepr+ versus Glp1r+, UMW = 5017, PuT = 0.001; Nmur2: Lepr+, Q1: 475.4, Q2: 689.7, Q3: 1,057; Glp1r+ Q1: 407.3, Q2: 574.9, Q3: 852, Lepr+ versus Glp1r+, UMW = 8,760, PuT = 0.0029. In b and c, solid white lines represent the median (Q2) and dashed white lines represent lower and upper quartiles (Q1 and Q2, respectively). P values were calculated using the unpaired Mann–Whitney (MW) U-test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ***P ≤ 0.0001.

Back to article page