Extended Data Fig. 2: MCH enhances excitatory transmission in dLS-to-LHA projecting neurons via a presynaptic mechanism.
From: Hypothalamic melanin-concentrating hormone regulates hippocampus-dorsolateral septum activity
a, Quantification of mRNAs of MCHR1 or vGAT in the dLS.n = 10. b, Sample traces of sEPSCs before and after trains of optogenetic activation of MCH fibers (left panel); right panel, Pooled data of the comparisons of frequencies and amplitudes of sEPSCs. n = 15 cells/8 animals and 16/9 cells/animals, at 5 Hz and 20 Hz, respectively. Paired Student’s t-tests were used: sEPSC frequency: Control vs. Post 5 Hz p = 0.0053; Control vs. Post 20 Hz p = 0.0051; sEPSC amplitude: Control vs. Post 5 Hz p = 0.0533; Control vs. Post 20 Hz p = 0.047. c, Sample traces of sEPSCs before and after optogenetic activation of MCH fibers in the presence of SNAP94847; right panel, Pooled data of the comparisons of frequencies and amplitudes of sEPSCs. n = 7 cells/3 animals and 10/4, at 5 Hz and 20 Hz, respectively. Student’s t-tests were used: sEPSC frequency: Control vs. Post 5 Hz p = 0.3281; Control vs. Post 20 Hz p = 0.5684; sEPSC amplitude: Control vs. Post 5 Hz p = 0.6994; Control vs. Post 20 Hz p = 0.8967. d&e, In the presence of Tc-MCH7c, no changes in spontaneous (d) or evoked EPSCs (e) were observed post 20 Hz optogenetic stimulation of MCH fibers.n = 9 cells /4 animals (d), 12/4 (e). Student’s t-tests were used: d, sEPSC frequency, Control vs. Post 20 Hz p = 0.584; sEPSC amplitude, Control vs. Post 20 Hz p = 0.9283; e, eEPSC amplitude, Control vs. Post 20 Hz p = 0.2007; PPR, Control vs. Post 20 Hz p = 0.4978. Data are mean ± SEM, * p < 0.05, ** p < 0.01, ***p < 0.001.
