Extended Data Fig. 8: Task representations show increased stability with learning following a break in training.

a. In the 2-odor task, Late and Reacquisition sessions were separated by multiple extinction sessions. To assess how task representations may change across a similar time period, but with no additional task experience, following learning of the 4-odor task, mice were kept in their homecage and rerun on the learned task 4 days later (Post). b. Mean lick rate during the trace period for all animals (n = 8 vCA1, 5 dCA1 mice,, two sided Mann-Whitney U test, * p < 0.05, *** p < 0.001, error bars mean ± SEM). c, d. Trial-type and CS+ vs CS- decoding accuracies were similar for the Post session (shown here) compared to Late (Fig. 5c and Extended Data Fig. 7a, b; Analyses used 150 cells for each region). e.As in Late session, odor and outcome information were multiplexed in vCA1 during the odor delivery period, while outcome information was present in both vCA1 and dCA1 during trace (n = 10 decoding iterations from n-matched 150 cells from 8 vCA1 and 5 dCA1 mice, two sided Mann-Whitney U test, *** p < 0.001, error bars are mean ± SEM). f. Pearson’s correlation of activity patterns across time bins. g. Task representations showed greater stability once learned. Analyses used cells registered across all 3 sessions. (n = 10 decoding iterations from n-matched 100 cells from 8 vCA1 and 5 dCA1 mice, two sided Mann-Whitney U test, * p < 0.05, ** p < 0.01, *** p < 0.001, error bars are mean ± SEM). h. Same as in g, but decoding CS+ vs CS- across sessions. See Supplementary Table 1 for all statistical analysis details.