Extended Data Fig. 9: SNc somas of genetic dopamine neuron subtypes have similar signaling patterns to their axons in response to rewards and air puffs and during locomotion.
From: Unique functional responses differentially map onto genetic subtypes of dopamine neurons
(a-e) Same as Fig. 4 but for recordings made in SNc. (a) ΔF/F averages triggered on reward delivery times for all recordings of each subtype and DAT. Isosbestic control shown in light blue, same scale as ΔF/F average. Acceleration shown in gray in the background (scale bar = 0.2 m/s 2). Shaded regions denote mean ± s.e.m. Heatmap shows triggered average for each recording, sorted by size of reward response. Vglut2 mice = 9, n = 25 recordings; Calb1 mice = 5, n = 10; Anxa1 mice = 5, n = 23; Aldh1a1 mice = 11, n = 40; DAT mice = 7, n = 39. (b) ΔF/F averages triggered on air puff delivery times for all recordings of each subtype and DAT. Isosbestic control shown in light blue, same scale as ΔF/F average. Acceleration shown in gray in the background (scale bar = 0.2 m/s 2). Shaded regions denote mean ± s.e.m. Heatmap shows triggered average for each recording, sorted by reward size as in A. Vglut2 mice = 9, n = 25 recordings; Calb1 mice = 5, n = 10; Anxa1 mice = 5, n = 25; Aldh1a1 mice = 11, n = 41; DAT mice = 8, n = 47. (c) Average reward and air puff responses for each subtype. Error bars denote ± s.e.m. p-values for reward: Vglut2 = 5 × 10−05, Calb1 = 0.007, Anxa1 = 1 (not significant), DAT = 2 × 10−07. p-values for air puff: VGlut2 = 5 × 10−05, Calb1 = 0.008, Anxa1 = 1 (not significant), DAT = 3 × 10−05. Two-sided Wilcoxon sign-rank test with Bonferroni correction. Same recordings and n as A,B. (d) Reward vs air puff responses for all recordings of each subtype and DAT. X shows mean for each subtype. Shaded regions are areas representing greater air puff than reward response (for Vglut2) or greater reward vs air puff response (for Calb1). (e) Comparison of responses to small vs large rewards for each subtype. Error bars denote mean ± s.e.m. p-values: Vglut2 = 0.05 (not significant), Calb1 = 0.03, Anxa1 = 1 (not significant). Two-sided paired Wilcoxon Signed Rank test with Bonferroni correction. Vglut2 mice = 9, n = 25 recordings; Calb1 mice = 5, n = 10; Anxa1 mice = 5, n = 23. (F) 3D plot showing locomotion (PC1/PC2 angle), reward and air puff responses for each recording and each subtype, comparing striatal recordings (same as Fig. 6a) and SNc recordings. (G) 2D plots for each pair of variables shown in the 3D plot in F. (h-k) Same as Fig. 2 but for recordings made in SNc. (h) Average cross-correlation between ΔF/F traces and acceleration for all recordings of each subtype. Isosbestic control shown in blue. Shaded regions denote mean ± s.e.m. Heatmap shows cross-correlation for each recording, sorted by PC1/PC2 angle (see Fig. 2l). Vglut2 mice = 11, n = 28 recordings; Calb1 mice = 3, n = 6; Anxa1 mice = 8, n = 34; Aldh1a1 mice = 13, n = 42; DAT mice = 8, n = 31. (I) ΔF/F averages triggered on large accelerations (left, ▲) and large decelerations (right, ▽) for all recordings of each subtype. Isosbestic control shown in light blue, same scale as ΔF/F average but shifted. Acceleration shown in gray in the background (scale bar = 0.2 m/s2). Shaded regions denote mean ± s.e.m. Heatmap shows triggered average for each recording, sorted as in H. (J) Acceleration averages triggered on large transients for all recordings of each subtype. ΔF/F average and isosbestic control shown in the background (scale bar = 5% Norm ΔF/F.) Shaded regions denote mean ± s.e.m. Heatmap shows triggered average for each recording, sorted as in H. (K) Principal component scores for each recording of each subtype along PC1 and PC2 (same PCs obtained from the striatal recordings, as shown in Fig. 2j–l). X shows mean for each subtype. Striatal PCs explain 77.6% of SNc variance (52.4% PC1, 25.2% PC2).
