Extended Data Fig. 2: Integration of scRNA-seq datasets reveals more granular resolution of DA neuron subtypes.
From: Unique functional responses differentially map onto genetic subtypes of dopamine neurons
(a) Resulting clusters from integrating datasets. (b) Expression patterns of Anxa1 and Aldh1a1, the top defining markers for cluster 1. Expression of Anxa1 appears to be limited to a subset of Aldh1a1-expressing neurons. (c) Violin plots of number of genes and RNA counts from each source dataset, which were used to determine cutoffs for quality control filtering. (d) LIGER clustering of the meta-dataset, revealing one cluster that was more distantly related to all other DA neurons and came solely from the Tiklova et al.26 dataset. This cluster was subsequently removed. (e) Cells colored by cluster (left) or source dataset (right), which reveals that all clusters were represented by each dataset. (f) Violin plots of the top 2 defining marker genes for each cluster.
