Extended Data Fig. 5: Confocal collection efficiency. | Nature Neuroscience

Extended Data Fig. 5: Confocal collection efficiency.

From: High-speed multiplane confocal microscopy for voltage imaging in densely labeled neuronal populations

Extended Data Fig. 5

Simulated confocal collection efficiency for fluorescence emitted from point sources (left) and 15-μm shells (right) located at different axial positions. Axial resolution is degraded as a result of spherical aberration induced by imaging through 0.5 mm cover glass (bottom; RI = 1.52). Lateral resolution is not degraded as much as axial resolution, meaning that the total fluorescence together collected by all detectors is only modestly reduced. See Methods and Supplementary Note 4 for more details.

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