Extended Data Fig. 2: System resolution. | Nature Neuroscience

Extended Data Fig. 2: System resolution.

From: High-speed multiplane confocal microscopy for voltage imaging in densely labeled neuronal populations

Extended Data Fig. 2

Experimental measurements of the combined excitation and emission point spread functions (PSFs)corresponding to the four simultaneously acquired image planes. Confocal pinhole diameters were selected so that PSFs corresponding to adjacent detectors overlap, eliminating gaps between the four imaging planes, which together cover ~40 μm in the axial direction. Lateral resolution was somewhat anisotropic on account of the slower detector fall time. (X: 2.6 μm full-width half-max; Y: 1.9 μm).

Back to article page