Extended Data Fig. 2: Bias analysis of (sub)clusters.

a. Normalized number of cells coloured by genoype for each cluster in resolution 8. Most clusters have a relatively similar number of cells from each genotype. b. tSNE of all cells coloured by genotype shows cells of different DGRP lines mix well. c, e. tSNE displaying cells colored by assignment to their run reveals that there are no technical batch effects for EB ring (c) and clock neuron (e) subclustering. d, f. Proportion of cells in each cluster colored by their assignment to their run for EB ring (d) and clock neuron (f) subclusters.