Extended Data Fig. 7: Mapping outputs of the Glu^S1HL and GABA^CeA neurons.

a, Schematic diagram of AAV-DIO-ChR2-EYFP injected into the S1HL of CaMK2-Cre mice. b, A representative confocal image of AAV-DIO-ChR2-EYFP expression in the S1HL. Scale bar, 100 µm. c, Representative confocal images of EYFP⁺ fiber expression in the CPu, S2, cp, PO, VPM and Cu of mice injected with AAV-DIO-ChR2-EYFP into the S1HL. Scale bar, 100 µm. d, Schematic diagram of AAV-DIO-ChR2-EYFP injected into the CeA of GAD2-Cre mice. e, Representative confocal image of AAV-DIO-ChR2-EYFP expression in the CeA. Scale bar, 100 µm. f, Representative confocal images of EYFP⁺ fiber expression in the BNST, PVN, VMH, LHb, PF and PAG of mice injected with AAV-DIO-ChR2-EYFP into the CeA. Scale bar, 100 µm. CPu, caudate putamen; S2, secondary somatosensory cortex; cp, cerebral peduncle; PO, posterior thalamic nuclear; VPM, ventral posteromedial thalamic nucleus; Cu, cuneate nucleus; BNST, bed nucleus of the stria terminalis; PVN, paraventricular thalamic nucleus; VMH, ventromedial hypothalamic nucleus; LHb, lateral habenular nucleus; PF, parafascicular thalamic nucleus; PAG, periaqueductal gray.