Extended Data Fig. 1: Neuropathic pain-induced changes in splenic immune cell populations. | Nature Neuroscience

Extended Data Fig. 1: Neuropathic pain-induced changes in splenic immune cell populations.

From: Somatosensory cortex and central amygdala regulate neuropathic pain-mediated peripheral immune response via vagal projections to the spleen

Extended Data Fig. 1

a, Gating strategy of the different immune cell subpopulations in the splenic immune cells analyzed by flow cytometry. b–d Flow cytometry dot plots (left) and summary data (right) of B cells (b), T cells (c), and NK cells (d) in the spleen of 1D, 1W, 2W, 3W, and 4W SNI mice (and time-matched sham mice). e, Flow cytometry dot plots (left) and summary data (right) of CD8 T cells in the spleen of 1D, 1W, 2W, 3W, and 4W SNI model mice (and time-matched sham mice). f, Flow cytometry dot plots (left) and summary data (right) of CD4 T cells in the spleen of 1D (t24 = 2.167, P = 0.0404), 1W (t19 = 0.4459, P = 0.6607), 2W (t11 = 1.643, P = 0.1287), 3W (t10 = 0.4361, P = 0.6721) and 4W (t23 = 3.878, P < 0.001) SNI mice (and time-matched sham mice). Significance was assessed by two-tailed unpaired Student’s t-test in (b–f). All data are presented as the mean ± SEM. *P < 0.05, ***P < 0.001, not significant (n.s.). Details of the statistical analyses are presented in Supplementary Table 1.

Back to article page