Extended Data Fig. 8: Modeling oligodendrocyte growth, loss, and regeneration in adult mouse cortex and white matter.
a, b) Cumulative oligodendrocyte population growth (% gain over time) in the healthy brain was modeled using asymptote-restricted exponential mechanistic growth curve-fitting. a) Cumulative OL gain (%) and mechanistic growth fit in the gray matter (left) and the white matter (right) for an example mouse. b) Cumulative OL gain (%) and mechanistic growth fits in the gray matter (left) and the white matter (right) for the healthy group (n = 6 mice). c, d) Cumulative oligodendrocyte population loss (% loss over time) due to cuprizone administration was modeled using three-parameter Gompertz Sigmoid curve-fitting. c) Cumulative OL loss (%) and three-parameter Gompertz curves in the gray matter (left) and the white matter (right) for an example mouse. d) Cumulative OL loss (%) and three-parameter Gompertz curves in the gray matter (left) and the white matter (right) for the cuprizone de/remyelination group (n = 6 mice). e, f) Cumulative oligodendrocyte population regeneration (% cell replacement over time) following cuprizone cessation was modeled using three-parameter Gompertz Sigmoid curve-fitting. e) Cumulative OL replacement (%) and three-parameter Gompertz curves in the gray matter (left) and the white matter (right) for an example mouse. f) Cumulative OL replacement (%) and three-paramter Gompertz curves in the gray matter (left) and the white matter (right) for the cuprizone de/remyelination group (n = 6 mice). Modeled rate and timing metrics in Main Figs. 4–7 were calculated by fitting curves to data from individual mice and then extracting summary data (for example timing of inflection point).
