Extended Data Fig. 2: GRIN lens implant and cell labeling verification.
From: Population-level coding of avoidance learning in medial prefrontal cortex
(a) Verification of the placement of the GRIN lens, with the GRIN lens position displayed in an example coronal mouse brain section. Nissl stain (red) and GCaMP6m (green). The shade blue area below the GRIN lens corresponds to an estimate of the distance between the edge of the implant (displayed in b) and the imaging plane (200 μm). (b) Locations of the edge of the implant were mapped onto the mouse brain atlas (see ref. 44) for each individual animal. (c) Left: immunohistological validation of GCaMP6m expression in PL, comparing GCaMP6m (green) and neurogranin labeling (red) in excitatory neurons. Top right: overlap. Middle right: GCaMP6m. Bottom right: neurogranin. (d) Left: comparison of GCaMP6m expression (green) to GAD65 staining (red). Top right: overlap. Middle right: GCaMP6m-expressing neurons. Bottom right: GAD65-positive neurons. Arrows indicate three examples of GAD65-positive neurons. Five independent repetitions with images from different subjects produced qualitatively similar results (c,d).
