Extended Data Fig. 7: Song type selective signals in the circuit are carried by a genetically defined population of neurons.
From: Activity of nested neural circuits drives different courtship songs in Drosophila
a, Example images of the calcium signals recoded from the dorsal (left) and medial (right) parts of the VNC. Images represent averages of frames across z-planes and time. Scale bars, 50 μm. b, Changes in calcium signals across song-type transitions for the example fly shown in Fig. 5b. Images were averaged across z-planes and time. Left, average frames during pulse to sine transitions (n = 137 transitions). Right, average frames during sine to pulse transitions (n = 65 transitions). Scale bars, 50 μm. c, Population averaged changes in ΔF/F during song-type transitions for the recordings from the dorsal part of the VNC for fru-expressing neurons (n = 6 flies). Scale bars, 50 μm. d,e, Same as b,c but for the recordings from the medial part. (d) n = 142 and 68 pulse-to-sine and sine-to-pulse transitions, respectively. (e) n = 5 flies. f–n, Calcium imaging of non-fru-expressing neurons. f, GCaMP was expressed using the pan-neuronal driver while its expression was suppressed in fru-expressing neurons. g,h, Same as b,c but for non-fru-expressing neurons. (g) n = 34 and 13 pulse-to-sine and sine-to-pulse transitions, respectively. (h) n = 5 flies. i,j, Same as d,e but for non-fru-expressing neurons. (i) n = 32 and 12 pulse-to-sine and sine-to-pulse transitions, respectively. (j) n = 3 flies. k, Population averaged ΔF/F for the difference between pulse and sine song around song-type transitions for the recordings from the dorsal part of the VNC for non-fru-expressing neurons (Left, n = 5 flies). The pan-neuronal imaging result described in Fig. 4d is also shown for comparison (Right). l, Histograms of the mean difference in ΔF/F between pulse and sine song during song-type transitions for voxels that changed ΔF/F depending on song-type (P < 0.001, two-sided permutation test). Left, non-fru-expressing neurons (n = in 5 flies). Right, the pan-neuronal imaging result shown in Fig. 4e. m,n, Same as k,l but for the recordings from the medial part (n = 3 flies).
