Fig. 6: Calcium dynamics of parallel descending pathways during the singing of pulse and sine song.
From: Activity of nested neural circuits drives different courtship songs in Drosophila
a, Schematic of calcium imaging in the brain during fly singing. b, Expression patterns of the pIP10 split-Gal4 (left) and the pMP2 split-Gal4 (right). The confocal stacks for the pIP10 split-Gal4 and pMP2 split-Gal4 were from ref. 17 and ref. 24, respectively. Similar expression patterns were observed in three flies for each genotype. Scale bars, 50 μm. c, Example ΔF/F trace of pIP10 neurons (top) together with the simultaneously recorded sound (bottom). Calcium signals reflect combined activity of the left and right pIP10 neurons. d, Time course of pIP10 ΔF/F (top) and song (bottom) around song-type transitions. Dashed vertical lines represent the timing of transitions. Data are from 9 flies and represented as mean ± s.e.m. across transitions for both ΔF/F and song (n = 2,827 and 1,145 events for pulse-to-sine and sine-to-pulse transitions, respectively). e, The mean change in ΔF/F after song-type transitions relative to ΔF/F before the transitions (see Methods for details) for pIP10. Each dot represents a neuron. Lines represent mean ± s.d. across neurons (n = 9 flies). P = 0.21 (pulse-to-sine transitions); P = 0.020 (sine-to-pulse transitions); two-sided one-sample t-test with Bonferroni correction. f–h Same as c–e but for pMP2. Calcium signals were recorded from neurites of individual pMP2 neurons. g, n = 1,487 (pulse-to-sine) and 524 (sine-to-pulse) transitions from 12 neurons in 7 flies. h, n = 12 neurons in 7 flies. P = 0.0012 (pulse-to-sine transitions); P = 0.0027 (sine-to-pulse transitions). See also Extended Data Fig. 8.
