Extended Data Fig. 7: Astrocyte monocultures require Tau for proper LD formation during stress.
From: Tau is required for glial lipid droplet formation and resistance to neuronal oxidative stress
(a) HBSS was used to induce stress in rat astrocytes grown in the absence (monoculture, n = 6) or presence (co-culture, n = 5) of neurons. LDs were then stained in these astrocytes using BODIPY 493/503. A datapoint represents the average LD number or size from 10 glia per experimental replicate (n). (b) WB showing GFP (control) or GFP-hTau expression in astrocyte monocultures prior to co-culturing with neurons. (c-e) LipidTox was used to assess LD number in astrocyte monocultures. CM = complete glia media (control). Glia were treated with HBSS to induce oxidative stress19. A datapoint represents the average LD number from 5-10 glia per experimental replicate (n). n = 3-4. (c) Astrocytes were transduced to overexpress GFP (control) or GFP-hTau. (d-e) Astrocytes were transduced to express RNAi targeting Mapt to a control RNAi. GFP marks cells that were successfully transduced. Statistics: unpaired 2-sided student t-test (a), 1-way Anova with Tukey’s multiple comparisons test (b and c). P-values = not significant (n.s.) >0.05, *≤0.05, **≤0.01, ***≤0.001, ****≤0.0001. Data presented as mean ± SEM. See Supporting Data 1 for details on n and P-values. All images are maximum intensity projections.
