Fig. 1: Multimodal transcriptomics of the mouse hippocampal neurogenic niche during aging.
a, Schematic illustration summarizing experimental design of the multimodal transcriptional atlas of mouse hippocampal neurogenic niche. b, UMAP visualization of all DG resident cells in the scRNA-seq dataset. c, Hierarchical clustering of all DG resident cells showing distinct transcriptional profiles among all 11 cell types. d, Feature plots of selected genes showing cell-type-specific expression profiles of all DG resident cells. Color gradient indicates the log-normalized gene expression level. e, Relative proportions of all cell populations in the mouse DG. f, UMAP visualization of all spots of the whole mouse brain in the ST dataset. g, Spatial projection of all spots into each age. h, Spatial mapping of annotated hippocampal cell populations from the Yao et al.35 dataset to the current ST dataset using the Seurat CCA tool. Computed enrichment score of each cell type (color gradient) to hippocampus shown over the H&E images. CCA, canonical correlation analysis; C–R, Cajal–Retzius; MO, months old; OPC, oligodendrocyte precursor cell; PN, pyramidal neuron; SMC, smooth muscle cell.
