Extended Data Fig. 2: Role of CLEC16A in the control of mitophagy in astrocytes.
From: CLEC16A in astrocytes promotes mitophagy and limits pathology in a multiple sclerosis mouse model
(a) Live cell imaging of siRNA KD FCCP or TNF/IL-1β-stimulated murine astrocytes, stained using Mitophagy dye (red) and Lyso-dye (green). Merged area indicate mitochondria undergoing mitophagy. Scale bars 100 μm. Regions of interest are highlighted with white squares and displayed in Fig. 2c. Representative of two experiments. (b, c) Live cell imaging of Control, Atg7 or Clec16a siRNA KD and TNFα/IL-1β-stimulated murine astrocytes, stained using Mitophagy dye (red) and Lyso-dye (green). The astrocytes were also pre-stimulated with TNFα/IL-1β for 13 hrs. (b) Representative images are shown. High Mitophagy dye intensity area generated from the original image by cutting off the low intensity signal area is also shown. Scale bars 30 μm. (c) Quantification of the overlay puncta numbers or area size of the high mitophagy-dye intensity area and lyso-dye are calculated from 9 splitted fields per each time point in each condition. Mean and s.e.m. p** < 0.01, p*** < 0001, p**** < 0.0001 by One-way ANOVA Dunnett’s post-test. (No. overlay puncta siControl versus siAtg7 P = 0.0090, overlay puncta area size siControl versus siAtg7 P = 0.0002).
