Extended Data Fig. 7: Peripheral immune cells in CLEC16AGFAP EAE mice.
From: CLEC16A in astrocytes promotes mitophagy and limits pathology in a multiple sclerosis mouse model
(a) Flow cytometry analysis of CD4+ T cells (CD4+CD3+) and monocytes (CD45hiCD11b+Ly6chi) in CNS and spleen of Control and CLEC16AGFAP mice 30 days after EAE induction. (b) Flow cytometry analysis of IL-17A for Th17 cells, IFNγ for Th1 cells, Foxp3 for regulatory T cells, and IL-10 for type 1 regulatory T cells in CNS and spleen of EAE mice (day 30). (c) Recall cytokine response of splenocytes from Control and CLEC16AGFAP mice (day 30) to MOG(35-55). (a-c) Mean and s.e.m. ***p < 0.001, **p < 0.01, *p < 0.05 by Mann Whiney test, two-sided (CD4+ T cells p 0.0022, monocytes p 0.0022) (a) and two-way ANOVA Sidak post-test (IL-17 + IFNγ- p 0.0007, IL-17-IFNγ + p 0.0353) (b). Representative of two experiments. (a, b) In the box plots, the data are presented as the median and interquartile range and whiskers represent minimum to maximum.
