Extended Data Fig. 1: Analysis of the mitophagy-related and mitochondrial proteins.
From: CLEC16A in astrocytes promotes mitophagy and limits pathology in a multiple sclerosis mouse model
(a) PINK1 expression in Clec16a siRNA KD primary astrocytes stimulated with TNF/IL-1β. Representative of 3 experiments. (b) Parkin and mitophagy-specific receptor expression in Clec16a siRNA KD primary astrocytes. Representative blots and quantification of mean fold change from baseline (time 0) of normalized mitophagy-specific receptor expression from 3 experiments are shown. *p < 0.05 by paired t-test, two-sided (Calococo2 16Hours p 0.0131). (c) Expression of mitochondrial protein TOM20. Representative blots and quantification from 3 experiments are shown. Mean and s.e.m. p < 0.05 by two-way ANOVA, Sidak post-test (TNFα/IL-1β p 0.0123).
