Extended Data Fig. 9: Quantification of co-clustering between cortical glutamatergic neurons of mice, reptiles, and salamander grouped by areas and LISI scores.
(a) Broad quantification of co-clustering in the integrated clusters between glutamatergic neurons from mouse and non-mammalian cortical areas, highlighting greater transcriptomic similarity of piriform glutamatergic neurons to those of non-mammals than to those of the neocortex. Pir: piriform; NCx: neocortex; DCtx, LCtx: dorsal, lateral cortex; aDVR: anterior dorsal ventricular ridge; dDP, LP, dVP: deep dorsal, lateral, deep ventral pallium. Rectangles indicate co-clustering of neurons (rows) in the integrated clusters (columns). Color of the rectangle represents the percentage of neurons in the integrated cluster. (b) Distribution of LISI scores across neuronal clusters of the datasets Tosches et al.45 (turtle and lizard), Hain et al.43 (lizard), and Woych et al.42 (salamander). A mean value close to 4 indicates a cell type that is well-mixed with neurons from other species, while a value close to 0 indicates a cell type mixed only with neurons from the same species. Box-and-whisker plots show min to max, center (median), 25th and 75th percentile box bounds, and whiskers extending to 1.5 * interquartile range (see Supplementary Table 1 for sample size).
