Extended Data Fig. 7: Nonshock controls do not exhibit conditioning-associated dynamics.
From: Psilocybin-enhanced fear extinction linked to bidirectional modulation of cortical ensembles
a, Schematic of nonshock protocol. Three Miniscope-implanted mice underwent an identical 5-day paradigm to all other mice, with the exception that they received no shock during Acquisition or drug treatment. b, Half-session freezing in nonshock mice (one-way RM ANOVA with Sidak correction; Supplementary Table 7 (row 1)). c, Number of longitudinally registered neurons in nonshock mice. d, Sum of session discriminability index. Because roughly half the number of neurons were recorded in nonshock mice as in the other two groups, pooled tensors from psilocybin responders, nonresponders and saline mice were subsampled to a different, random set of 160 neurons in each of 100 iterations of TCA (F(3,297) = 6694, p < 0.0001, one-way RM ANOVA with Sidak correction; Supplementary Table 7 (row 2). e–g, Overlap of the (e) day 2-dominant ensemble with (f) day 3- and (g) day 5-dominant ensembles in nonshock mice. Bar graphs display the median fraction overlaps. Dots are individual animals. Insets are pie charts displaying total overlap. Stars indicate comparison to low-freezing saline distribution (Acq-dominant: chi square = 10.84, p = 0.0126; Ext1-dominant: chi square = 16.04, p = 0.0011; Ext3-dominant: chi square = 30.50, p < 0.0001; chi-square test; Supplementary Table 7 (rows 3–5)). h–j, Average z score with respect to day 2 of (h) day 2-, (i) day 3- and (j) day 5-dominant ensembles, respectively, during day 3 and 5 in nonshock mice (turquoise) compared to conditioned, saline-administered mice (black; Acq-dominant: F(group)(1,202) = 9.329, p = 0.0026; Ext3-dominant: F(interaction)(1,240) = 5.787, p = 0.0169; F(session)(1,240) = 23.06, p < 0.0001, F(group)(1,240) = 4.534, p = 0.0342; two-way RM ANOVA with Sidak correction; Supplementary Table 7 (rows 4–6)). Data are represented as mean ± s.e.m. *p ≤ 0.05, **p < 0.01, ****p < 0.0001.
