Fig. 4: Effects of ChP overstimulation on cortical and ganglionic eminence progenitor proliferation and differentiation.
From: Choroid plexus apocrine secretion shapes CSF proteome during mouse brain development
a, Schematic of experimental design. b, Diagram illustrating the analyzed regions of interest: cortex, LGE and MGE. c–e, Quantification and representative images of PHH3+DAPI+ mitotic nuclei per 10,000 µm² in the cortex (c; control N = 13; WAY-161503 N = 13), LGE (d; control N = 10; WAY-161503 N = 14) and MGE (e; control N = 10; WAY-161503 N = 13) at 2 h post-EdU. f, Quantification and representative images of EdU+ nuclei in cortical CP/IZ and VZ regions at 2 h post-EdU (control N = 13; WAY-161503 N = 15). g,h, Quantification and representative images of EdU+ nuclei per 10,000 µm² in the LGE (g; control N = 9; WAY-161503 N = 12) and MGE (h; control N = 9; WAY-161503 N = 12) at 2 h post-EdU. i–k, Quantification and representative images of PHH3+DAPI+ mitotic nuclei per 10,000 µm² in the cortex (i; control N = 10, WAY-161503 N = 11), LGE (j; control N = 9, WAY-161503 N = 12) and MGE (k; control N = 10, WAY-161503 N = 9) at 18 h post-EdU. l, Quantification and representative images of EdU+ nuclei in cortical CP/IZ and VZ regions at 18 h post-EdU (control N = 10, WAY-161503 N = 12). m,n, Quantification and representative images of EdU+ nuclei per 10,000 µm² in the LGE (m; control N = 10, WAY-161503 N = 11) and MGE (n; control N = 10, WAY-161503 N = 10) at 18 h post-EdU. o, Quantification and representative images of overall EdU+ cell density at P8 in the cortex (control N = 9, WAY-161503 N = 8). p, Bar graphs depicting the percentage of TBR1+EdU+ cells (center) and TBR1+EdU– cells (right) and representative images (left) of the S1 at P8 (control N = 8, WAY-161503 N = 8). All data are presented as mean ± s.e.m. P values were calculated by two-sided t-tests for c–e, g–k, m and n, two-way ANOVA with a Sidak correction for f and l and one-way ANOVA with a Tukey correction for p; scale bars, 50 μm (c–e and i–k), 100 μm (f–h and l–n) and 200 μm (o and p). All N indicate the number of mice collected from three separate litters. Panel a created with BioRender.com.
