Extended Data Fig. 6: Pure tone and complex sound responses of layer 2/3 neurons are similarly affected by the microablation of sound responsive neurons.

To assess how far pure tone and complex sound responses in the spared network may be differentially affected by the removal of sound responsive neurons, we run analogous analyses as shown in the corresponding main figures, however, split the data into the two categories of stimulus responses. It should be noted that this reduced the size of the dataset, introducing increased variability. Nevertheless, we observed largely similar effects for both categories of stimuli. a. Analogous to Fig. 2e, baseline-normalized Pearson correlations of population vectors averaged across diagonal elements (left) and non-diagonal elements (right), split into pure tones (blue) and complex sounds (red), for sound responsive ablation cohort. Two-way ANOVA, p ≥ 0.18 both between PT and CS stimuli and across days for both diagonal elements and non-diagonal elements;. Two-sided t-test between PT and CS stimuli with FDR correction across post-ablation days, p > 0.57 for both diagonal and non-diagonal elements. b. Analogous to Fig. 3a, normalized response reliability across trials for PT and CS stimuli. Two-way ANOVA, p = 0.91 between PT and CS tunings (See Supplementary Table 2.). Two-sided t-test of reliability between PT response vs. CS response with FDR correction across post ablation days (day 7, 9, 11, 15): p > 0.50. c. Analogous to Fig. 3c, fraction of responsiveness for PT and CS stimuli. Two-way ANOVA, p = 0.24 between PT and CS stimuli; p = 0.092 across days. Two-sided t-test of response fraction between PT response vs. CS response with FDR correction across post ablation days: p > 0.92. d. Analogous to Fig. 3d, maximum response amplitude for PT and CS stimuli in sound responsive neurons. Two-way ANOVA, p = 0.054 between PT and CS tunings; p = 4.69 × 10⁻⁸ across days. Two-sided t-test of maximum amplitude between PT response vs. CS response with FDR correction across post ablation days: p > 0.42. e. Analogous to Fig. 3e, normalized tuning curves for pure tones (left) and complex sounds (right) overlaid across days. Two-sided t-test with FDR correction between PT tuning vs CS tuning at 15th stimulus index on post-ablation days: p > 0.27. f. Stability of single-trial responses between PT and CS stimuli analyzed analogous to a previous study⁴³. Two-way ANOVA, p = 0.45 between PT and CS tunings; p = 2.13 × 10⁻¹² across days. Two-sided t-test of single-trial stability between PT and CS stimuli, on each day: p > 0.32. Data presented as mean ± s.e.m. across mice. n = 10 mice for sound responsive ablation for both types of sounds (pure tones, blue and complex sounds, red) for all panels.