Supplementary Figure 5: Locations of coiled-coil discontinuities in bacterial and archaeal Smc proteins.

a, Aggregate coiled-coil probability profile (same as in Fig. 5) and single-sequence profiles for B. subtilis Smc (bacterial) and Pyrococcus yayanosii Smc (archaeal). Positions of coiled-coil discontinuities experimentally determined by X-ray crystallography (Mol Cell 67, 334-347.e5, 2017) or disulfide cross-linking (Proteins 83, 1027–1045, 2015) are highlighted in red. b, The elbow region of P. yayanosii Smc. The predicted coiled-coil probability from aggregate analysis (see a and Fig. 5) is mapped onto the crystal structure of a central arm fragment (PDB ID 5XG2). Positions of the predicted and crystallographically determined discontinuities are shown.