Supplementary Figure 1: H1 inheritance patterns in Drosophila GSCs. | Nature Structural & Molecular Biology

Supplementary Figure 1: H1 inheritance patterns in Drosophila GSCs.

From: Asymmetric histone inheritance via strand-specific incorporation and biased replication fork movement

Supplementary Figure 1

(a) A schematic diagram showing the dual color switch design that expresses first preexisting histone and then newly synthesized histone by heat shock treatment, as adapted from2. (b) Histone H1 showed overall symmetric inheritance pattern in post-mitotic GSC-GB pairs (n=12). Individual data points (circles) and mean values are shown. Error bars represent 95% confidence interval. See Supplemental Table 1 for details. Neither old H1 nor new H1 is significantly different from the value of 1 based on two-tailed Wilcoxon signed-rank test. H1-GFP GSC/GB ratio = 1.26; H1-mKO GB/GSC ratio = 1.18. H1 old GSC/GB data: Shapiro-Wilk normality test P = 0.0069, data not normally distributed. Wilcoxon signed-rank test. Two-tailed test. Sum of signed ranks = 44. P = 0.0923. H1 new GB/GSC data: Shapiro-Wilk normality test P = 0.3147, data normally distributed. One sample t-test. Two-tailed test t = 1.546 df = 11. P = 0.1503. See Supplementary Tables 1 and 2 and online Methods for additional statistical information.

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