Extended Data Fig. 10: Relion 3D classification and local resolution of the full-length B. subtilis T-box−tRNA complex.
From: Structural basis of amino acid surveillance by higher-order tRNA-mRNA interactions
3D classification of the complex converged to three maps. Superposition of the tRNA density in three maps revealed motions of the T-box relative to tRNA as indicated by the arrows. This flexibility of the T-box RNA is the major limitation that prevented cryo-EM reconstruction from achieving higher resolutions. Resmap analysis shows that the tRNA was better resolved at ~4-Ã… resolution (upper right).
