Extended Data Fig. 2: Mutational analysis of T-box discriminator-tRNA interactions.
From: Structural basis of amino acid surveillance by higher-order tRNA-mRNA interactions
a, Secondary structures of wild-type, mutant, and truncated T-box discriminators. Deletions are indicated by red boxes. b, Electrophoretic mobility shift assay (EMSA) analysis of the constructs shown in a, showing the requirement of stem III and flanking purines for tRNA binding. The antiterminator (discriminator without stem III and its flanking purines; Δ3 mutant) is prone to dimerization. c, tRNA variants used that carry various 3′ chemical modifications. Only the terminal tA76 is shown. d, EMSA analysis of constructs in c, showing that binding is selective for uncharged tRNA. e, Quantitation of d and comparison with previously reported in vitro transcription readthrough data of the same tRNA variants. The values and error bars represent mean and s.d., n = 3 biologically independent samples.
