Extended Data Fig. 6: Interactions of MlaB to MlaF using AMP-PNP bound structure of MlaFEDB.
From: Structural insights into outer membrane asymmetry maintenance in Gram-negative bacteria by MlaFEDB
a, Cartoon representation of structure of the AMP-PNP bound MlaFEDB complex. b, 90° rotation along the y-axis relative to the left panel. c, Structure of MlaB showing the STAS ___domain, consisting of three α-helices and four β-strands. d, The interactions between one MlaB and one MlaF subunit. e, 90° rotation along the y-axis relative to the left panel. f, SDS-PAGE analysis of proteins of wildtype and MlaF binding residue mutants MlaFEDB(W29E), MlaFEDB(Y88E) and MlaFEDB(T52A). g, ATPase activity of Mutant MlaFEDB(T52A) in both detergent and liposomes. h, FRET scan of PL transport using mutant MlaFEDB(T52A) or wildtype MlaFEDB IM proteoliposomes in the presence of ATP and MgCl2 for retrograde direction. Data f, h are representative results from n = 3 independent experiments. Data in g, h presents mean ± s.d. (n = 3 independent experiments). Uncropped images for panel f are available online. Source data for panels g and h are available in Supplementary Data Set 1.
