Extended Data Fig. 1: Protein abundance for strains with heterozygous disruptions of HSP104 and/or RNQ1. | Nature Structural & Molecular Biology

Extended Data Fig. 1: Protein abundance for strains with heterozygous disruptions of HSP104 and/or RNQ1.

From: Nucleation seed size determines amyloid clearance and establishes a barrier to prion appearance in yeast

Extended Data Fig. 1

The relative levels of Hsp104 (a), Sup35 (b), and Rnq1 (c) in lysates from diploid yeast strains analyzed in Fig. 1e–g were determined by SDS-PAGE and immunoblotting with specific antisera. Strains carried wildtype (+/+), heterozygous (+/Δ), or homozygous (Δ/Δ) disruptions of HSP104 or RNQ1 and the indicated variant of [PSI+]. Protein levels are expressed relative to the protein levels in wildtype version of the same prion strain (that is [PSI+]Strong or [PSI+]Weak) in arbitrary units (a.u.). Data are mean and s.d. from 3 independent experiments performed with independent cultures. Data for the graphs in panels a-b are available as source data.

Source data

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