Extended Data Fig. 5: Sequence alignment of yeast Rad24, metazoan RAD17 and yeast Rfc1.
From: DNA is loaded through the 9-1-1 DNA checkpoint clamp in the opposite direction of the PCNA clamp
Helices and β-strands are shown as coils and arrows, respectively. The assignments are produced by ESPript 3.0 (https://espript.ibcp.fr/ESPript/ESPript/) based on the structures S.c. Rad24 (this study) and S.c. Rfc1 (PDB code 1SXJ). The flashy color scheme was applied. Key features are marked above the sequences. Note that the linker region between collar and AAA+ module of Rfc1 contains only one residue (L549) and is not included in the box. The SRC (serine-arginine-cysteine) motif – conserved in Rfc2–5 – is absent in Rad24/RAD17 and Rfc1/RFC1; its ___location corresponding to the SRC motif in Rfc2–5 is boxed and labeled as SRC*.
