Extended Data Fig. 6: Structural comparison of Rad24 and Rfc1/RFC1.
From: DNA is loaded through the 9-1-1 DNA checkpoint clamp in the opposite direction of the PCNA clamp
Upper panel, the electrostatic potential charge surface of the S.c. Rad24 (this study), S.c. Rfc1 (PDB code 1SXJ), and human RFC1 (PDB code 6VVO). In Rad24, a contiguous basic patch on top of the AAA+ ___domain enables DNA binding. Rfc1/RFC1 lack such contiguous basic path. Lower panel, the corresponding atomic models shown in cartoons. These structures are aligned based on the full loader complexes to achieve the best superposition. The four tandem basic residues in Rad24 and the corresponding region in Rfc1/RFC1 are highlighted in firebrick. Note that the lid and Rossmann fold domains move away from the collar ___domain plus the associated A’ ___domain by a 35-Å shift and a 20º rotation, creating a large groove for dsDNA binding.
