Extended Data Fig. 8: Comparison of the available structures of the DNA clamp–clamp loader–(DNA) complexes.
From: DNA is loaded through the 9-1-1 DNA checkpoint clamp in the opposite direction of the PCNA clamp
a) Structure of the yeast Rad24-RFC bound to 9-1-1 and DNA compared with the T4 phage clamp loader–clamp bound to a DNA substrate. b) Comparison of the yeast RFC–PCNA (PDB code 1SXJ) with the human RFC–PCNA (PDB code 6VVO), all in a clamp ring closed configuration. The Rad24-RFC–9-1-1 clamp is more compact than the yeast and human RFC–PCNA structures, with the 9-1-1 ring tilting 25° toward the Rad24-RFC loader. c) Structure comparison of the Rad24-RFC–9-1-1 clamp–DNA with the T4 clamp–clamp loader–DNA (PDB code 3U60), both in the open clamp ring conformation. Note the 27-Å DNA gate in the planar 9-1-1 clamp with the 10-Å DNA gate in the T4 spiral clamp. To facilitate comparison, the loader subunits are labeled A through E according to their physical ___location in the complex.
