Extended Data Fig. 10: In cell DMS reactivities and secondary structure predictions for dimer and monomer promoting mutants.
From: Short- and long-range interactions in the HIV-1 5′ UTR regulate genome dimerization and packaging
In cell DMS reactivities and secondary structure predictions obtained for dimer and monomer promoting mutants targeting the polyA-SL1 and PBS-SL1 interactions. DMS reactivities for each nucleotide position are show in the upper barchart. Shannon entropies are shown in lower chart. Upper arc plots show consensus structure. Lower arc plots show base pairing probabilities (green = 70-100%; blue=40-70%; yellow=10-40%; grey=5-10%). (a) Wild-type HIV-1 (b) dimer promoting mutant C84A-C85A folds into the canonical 5’UTR structure (c) Monomer promoting mutant U86G-A89C contains the polyA-SL1 interaction. (d) Dimer promoting mutant C218G folds into the structure containing SL1 (e) Monomer promoting mutant U220G-G221A folds into a structure containing the PBS-SL1 interaction.
