Extended Data Fig. 7: Functional validation of the double mutant (A226V and A227L).
From: Inactive and active state structures template selective tools for the human 5-HT5A receptor
a-b, BRET2 Gi1-activation assays and c-d, BRET1 β-arrestin2 recruitment of WT and double mutant (A226V and A227L) 5-HT5AR stimulated by eight ligands. See Supplementary Table 12 for fitted parameter values that represent mean ± SEM of N = 3 biological replicates.
