Extended Data Fig. 2: CryoEM analysis for 5-HT5AR-miniGo bound to 5-CT, Lisuride, and Methylergometrine.
From: Inactive and active state structures template selective tools for the human 5-HT5A receptor
For each of the respective agonist bound 5-HT5AR heterotrimeric complexes are shown: a, Histograms of defocus values for micrographs used in the single-particle analysis (see Table 2 for more details). b. Representative frame aligned micrograph. The experiment was repeated three times with similar results. c, Orientational distribution heat map. d, 2D plots of the gold-standard Fourier shell correlation (GSFSC) between half maps (black) and FSC between model and the B-factor sharpened map for respective refined model (red) as calculated by Phenix.mitrage. e, Local resolution heat-map calculated using the local windowed FSC method.
