Extended Data Fig. 7: Characterization of NIH3T3 cell line expressing epitope-tagged SMO.
From: A PKA inhibitor motif within SMOOTHENED controls Hedgehog signal transduction
The NIH3T3 cell line used in Fig. 5 exhibited trace amounts of SMO in cilia under vehicle (‘Ctrl’) -treated conditions, and a dramatic accumulation of SMO in cilia under SAG-treated conditions. SMO (magenta) is visualized using an anti-V5 antibody, and cilia are visualized using an anti-Arl13b antibody (green). Scale bar = 10 µm. Data shown are representative of two independent experiments.
