Extended Data Fig. 2: Cryo-EM structure determination and analysis of PIC-nucleosome complex A.
From: Structures of transcription preinitiation complex engaged with the +1 nucleosome
a. Exemplary cryo-EM micrograph. A scale bar is provided. In total 26,764 micrographs were collected with similar results. b. Particle sorting and classification tree. Regions corresponding to Pol II, general transcription factors (GTFs) and template DNA are colored as in Fig. 2a, histones are colored in light yellow. Maps deposited to EMDB are indicated with grey background and outlined in black. The subpopulation of TBP-nucleosome complex is not described in this study since a similar TBP-NCP structure has been published before24. c. Representative 2D class averages of sorted particles used for final reconstruction.
