Fig. 1: Structure of the SA2–SCC1–SGO1 complex.

a, Structure of the SA2–SCC1–SGO1 complex. SA2 (blue), SCC1 (green) and SGO1 (yellow). b, Domain architecture and conservation of the YxF motif in SGO1. c, Crystal structure (left) and AlphaFold model (right) of the SA2–SCC1–SGO1 complex. Details of the CES binding pocket showing the interactions of SGO1 F337 and Y335. AlphaFold model: SGO1 amino acids spanning 341–349 including pT346 are predicted to form additional interactions with SA2. d, ITC experiments with SGO1 amino acids 331–341 SNDAYNFNLEE (left) and 331–349 SNDAYNFNLEEGVHLpTPFR containing phosphorylated pT346 (right). e, GST pulldown analysis of SGO1 and SA2 or SCC1 variants. M, molecular weight marker; I, input; B, bound fractions, analyzed by SDS polyacrylamide gel electrophoresis. Controls are shown in lanes 1 and 2. The experiment was repeated three times with consistency. WT, wild type.