Extended Data Fig. 4: OGT and OGA distribution during the cell cycle progression.
From: H4S47 O-GlcNAcylation regulates the activation of mammalian replication origins
a, b, Examination of proteins in cells synchronized at different stages of the cell cycle. HEK293T cells synchronized at G1/S were released and collected at indicated time points. Experimental setup and FACS profiles depicting the cell cycle progression were demonstrated (top). Both total proteins (a) and chromatin pellets (b) were analyzed by WB (bottom). Coomassie-stained total histones, H4 and β-actin served as loading controls. Asyn, asynchronization. H4S47ph, H4 phosphorylated at S47.
