Extended Data Fig. 5: Establishment of a model for the rapid degradation of MLLAF9.
From: CRISPR–ChIP reveals selective regulation of H3K79me2 by Menin in MLL leukemia
a) Kaplan-Meier curve of mice transplanted with MLLAF9FLAG-mAID cells or Nras + MLLAF9FLAG-mAID. b) Genome browser snapshot of MLLAF9 ChIP-seq replicates at the MEIS1 locus. FLAG ChIP from non-FLAG tagged MLLAF9 cells used as a negative control and input shown. c) Genome browser snapshot of MLLAF9 ChIP-seq replicates at the HOXA cluster. FLAG ChIP from non-FLAG tagged MLLAF9 cells used as a negative control and input shown. d) Proliferation assay of MLLAF9FLAG-mAID Tir1 cells treated with DMSO or IAA (500uM) over 7 days. Eror bars represent mean +/− SD from three independent biological replicates. e) FACS analysis of cell surface Gr1 levels in unstained, stained, DMSO or IAA treated MLLAF9FLAG-mAID Tir1 cells for 4 days. f) Profile plot of MLLAF9 FLAG ChIP-seq in MLLAF9FLAG-mAID cells treated with DMSO, IAA (2hrs and 4hrs). g) Heatmap of 4su-seq (nascent RNA-seq) in MLLAF9FLAG-mAID Tir1 cells treated with DMSO or IAA for 2hrs, showing 260 downregulated genes. h) PCA plot of RNA-seq from MLLAF9FLAG-mAID Tir1 cells treated with DMSO or IAA (2hrs). i) Heatmap of RNA Pol II ser2 ChIP-seq at all genes in MLLAF9FLAG-mAID Tir1 cells treated with DMSO or IAA (2hrs). j) Genome browser snapshots of MLLAF9 targets, EPHA7 and BAZ2B showing RNA-Pol IIS2ph and MLLAF9 ChIP-seq tracks after treatment with either DMSO or IAA (2hrs).
