Extended Data Fig. 10: PDHE1α is a critical mediator of cellular resistance to DNA-damaging treatment.
From: PARylated PDHE1α generates acetyl-CoA for local chromatin acetylation and DNA damage repair
a, Representative images of colony-formation corresponding to Fig. 7c. b, Genotyping of the Pdha1f/f and Pdha1f/f; CMV cre mice. The DNA from mice tail was extracted and subjected to PCR amplification using specific primers. c, Average body weight of mice post-IR (5 Gy). The body weight of each mouse was monitored every 4 days, and the curve was generated from the average weight of 10 mice per group. Data are mean ± s.d. d, The villi heights and average number of crypts per millimeter of the small intestine length were counted and plotted from 10 mice and each mice collected 2 views for analysis. The statistical analyses were performed using a two-tailed student’s t-test, and data shows the mean ± s.d. e, The intensity of indicated DNA damage markers (7K) were quantified. n = 3 biological replicates from the 10 mice was collected and analyzed using a two-tailed student’s t-test, data are mean ± s.d. f, PDHE1α-KO cells stably expressing PAR-binding-defective constructs were inoculated subcutaneously into nude mice. The average volume of each tumor and mouse body weight were monitored at the indicated time-points and used to plot the growth curve. g, PDHE1α-KO cells stably expressing PARylation-defective constructs were inoculated subcutaneously into nude mice, and the tumor site was irradiated every 5 days. The average body weight of each mouse was monitored at the indicated time-points. Data in f and g were collected from 8 mice of each group. h, H-scores of the indicated markers were quantified for three individual tumors collected from the 8 mice in each group. The statistical analyses were performed using a two-tailed student’s t-test, and data shows the mean ± s.d.
