Extended Data Fig. 5: Crosslinking assays to identify the TRIM72 oligomer.
From: Structure and activation of the RING E3 ubiquitin ligase TRIM72 on the membrane
a, Overall scheme of the time-dependent crosslinking assay. b-e, Time-dependent crosslinking analysis of mouse TRIM72 WT (b) and variants (c-e). Note that each variant of K2D (c), R3E (d), and ΔH3 (e) forms almost no high-molecular-weight crosslinked oligomers. All PS-liposomes contained 30 mol% PS.
