Extended Data Fig. 6: Higher-order TRIM72 assembly on both microvesicles and liposomes.
From: Structure and activation of the RING E3 ubiquitin ligase TRIM72 on the membrane
a, Time-dependent crosslinking analysis of TRIM72 WT purified from mammalian cells. b, SEC profiles of TRIM72 expressed in mammalian cells (blue line) and in vitro-reconstituted TRIM72 proteoliposomes (red line). The fractions containing TRIM72 bound to microvesicles or liposomes and TRIM72 in solution are indicated by arrows colored green and pink, respectively. c, Negative-stained TEM images of TRIM72 bound to microvesicles at low (left) and high magnifications (right). d, TEM images of the negatively stained TRIM72 proteoliposomes. (c, d) Higher-order TRIM72 assemblies are indicated by black arrowheads. The red and black scale bars indicate 100 and 20 nm, respectively. e, Cryo-electron tomograms (left) visualized by 3D volume rendering (right) showing the higher-order TRIM72 assembly on the inside convex (up) and outside concave (down) sides of small liposomes. The TRIM72 assembly and liposomal membranes are colored cyan and white, respectively. The black scale bars indicate 20 nm in e.
