Extended Data Fig. 1: Cryo-EM structure determination of Rpd3S-nucleosome complex.
From: Structure of histone deacetylase complex Rpd3S bound to nucleosome
a, SDS–PAGE of peak fraction of Rpd3S in complex with di-nucleosome used for cryo-EM grid preparation. Experiments were performed one time. For gel source data, see Supplementary Fig. 1. b, A representative cryo-EM micrograph showing particles of Rpd3S-nucleosome complex. Experiments were performed at least three times. c, Representative 2D class averages of the complex particles exhibiting major conformational heterogeneity. d, Sorting and classification tree used to reconstruct Rpd3S-di-nucleosome complex. e, Sorting and classification tree used to reconstruct Rpd3S-mono-nucleosome complex. Masks applied during classification and refinement are depicted as translucent outlines and specified in the accompanying text in blue. Complex subunits and submodules are highlighted according to the color codes in Fig. 1. Final reconstructions that were deposited to the EMDB are boxed with a light grey background and outlined in black.
